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Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods.

Abstract:

Background

Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia, and the commonest cause of mosquito-borne encephalitis worldwide. Detection of JEV RNA remains challenging due to the characteristic brief and low viraemia, with 0–25% of patients positive, and the mainstay of diagnosis remains detection of anti-JEV IgM antibody.

Methods

We performed a systematic review of published RT-PCR protocols, and evaluated them in silico and in vitro alo...

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Publication status:
Published
Peer review status:
Peer reviewed

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Role:
Author
ORCID:
0000-0002-6772-2855
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Name:
Horizon 2020
Grant:
653316
More from this funder
Name:
Institute of Research for Development
Publisher:
Public Library of Science
Journal:
PLoS One More from this journal
Volume:
13
Issue:
3
Pages:
e0194412
Publication date:
2018-03-23
Acceptance date:
2018-03-02
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
Pmid:
29570739
Language:
English
Keywords:
Pubs id:
pubs:832042
UUID:
uuid:e7a17e6f-4054-4963-b244-8a577823e744
Local pid:
pubs:832042
Source identifiers:
832042
Deposit date:
2018-04-04

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