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GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

Abstract:

The tetrameric GINS complex, consisting of Sld5-Psf1-Psf2-Psf3, plays an essential role in the initiation and elongation steps of eukaryotic DNA replication, although its biochemical function is unclear. Here we investigate the function of GINS in fission yeast, using fusion of Psf1 and Psf2 subunits to a steroid hormone-binding domain (HBD) to make GINS function conditional on the presence of beta-estradiol. We show that inactivation of Psf1-HBD causes a tight but rapidly reversible DNA repl...

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Publication status:
Published

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Publisher copy:
10.1091/mbc.e08-04-0429

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Journal:
Molecular biology of the cell
Volume:
20
Issue:
4
Pages:
1213-1222
Publication date:
2009-02-05
DOI:
EISSN:
1939-4586
ISSN:
1059-1524
URN:
uuid:e6b2834e-5449-4fb4-8947-caa00f38b03f
Source identifiers:
209224
Local pid:
pubs:209224

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