Journal article
Nitrate enhancement of CAM activity in two Kalanchoë species is associated with increased vacuolar proton transport capacity
- Abstract:
- Among species that perform CAM photosynthesis, members of the genus Kalanchoë have been studied frequently to investigate the effect of environmental factors on the magnitude of CAM activity. In particular, different nitrogen sources have been shown to influence the rate of nocturnal CO2 fixation and organic-acid accumulation in several species of Kalanchoë. However, there has been little investigation of the interrelationship between nitrogen source (nitrate versus ammonium), concentration and the activity of the vacuolar proton pumps responsible for driving nocturnal organic-acid accumulation in these species. In the present study with Kalanchoë laxiflora and Kalanchoë delagoensis cultivated on different nitrogen sources, both species were found to show highest total nocturnal organic-acid accumulation and highest rates of ATP- and PPi-dependent vacuolar proton transport on 2.5 mM nitrate, whereas plants cultivated on 5.0 mM ammonium showed the lowest values. In both species malate was the principal organic-acid accumulated during the night, but the second-most accumulated organic-acid was fumarate for K. laxiflora and citrate for K. delagoensis. Higher ATP- and PPi-dependent vacuolar proton transport rates and greater nocturnal acid accumulation were observed in K. delagoensis compared with K. laxiflora. These results show that the effect of nitrogen source on CAM activity in Kalanchoë species is reflected in corresponding differences in activity of the tonoplast proton pumps responsible for driving sequestration of these acids in the vacuole of CAM-performing cells.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 1.9MB, Terms of use)
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(Supplementary materials, zip, 8.8MB, Terms of use)
-
- Publisher copy:
- 10.1111/ppl.12578
Authors
- Publisher:
- Wiley
- Journal:
- Physiologia Plantarum More from this journal
- Volume:
- 160
- Issue:
- 4
- Pages:
- 361-372
- Place of publication:
- Denmark
- Publication date:
- 2017-06-02
- Acceptance date:
- 2017-03-05
- DOI:
- EISSN:
-
1399-3054
- ISSN:
-
0031-9317
- Pmid:
-
28393374
- Language:
-
English
- Pubs id:
-
690887
- Local pid:
-
pubs:690887
- Deposit date:
-
2020-03-31
- ARK identifier:
Terms of use
- Copyright holder:
- Scandinavian Plant Physiology Society
- Copyright date:
- 2017
- Rights statement:
- © 2017 Scandinavian Plant Physiology Society
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Wiley at https://dx.doi.org/10.1111/ppl.12578
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