Methods for monitoring and measurement of protein translation in time and space

Journal article

Regulation of protein translation constitutes a crucial step in control of gene expression. In comparison to transcriptional regulation, however, translational control has remained a significantly under-studied layer of gene expression. This trend is now beginning to shift thanks to recent advances in nextgeneration sequencing, proteomics, and microscopy based methodologies which allow accurate monitoring of protein translation rates, from single target messenger RNA molecules to genome-wide scale studies. In this review, we summarize these recent advances, and discuss how they are enabling researchers to study translational regulation in a wide variety of in vitro and in vivo biological systems, with unprecedented depth and spatiotemporal resolution.The authors are funded by a Medical Research Council (MRC) Career Development Award to F. K. M. (MR/P009417/1)

Authors: Dermit, M; Dodel, M; Mardakheh, FK

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Oxford University Press (OUP)
• Journal: Molecular bioSystems
• Volume: 13
• Issue: 12
• Pages: 2477-2488
• Publication date: 2017-10-12
• DOI: 10.1039/c7mb00476a
• EISSN: 1742-2051
• ISSN: 1742-2051
• Language: English
• Keywords: Translation (biology); Biology; Gene; Genetics; Computational biology; Computer science; Messenger RNA; Protein biosynthesis