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Bacteriorhodopsin/phospholipid interactions - a study by ³¹P- and ²H-NMR

Abstract:

Two methods were used to produce exogenous lipid/bR complexes. A detergent method (Huang et al., 1980) reconstituted bR into DMPC or DMPG bilayers, free of all endogenous purple membrane lipids as shown by high resolution 31 P-NMR. A novel biological detergent-free method employed bovine liver non-specific lipid transfer protein (nsTP) to mediate addition of DMPC to purple membrane, while retaining 76 - 86% of the endogenous purple membrane phospholipids.

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Institution:
University of Oxford
Department:
Life and Environmental Sciences Division
Role:
Author

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Supervisor
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Supervisor
Role:
Supervisor
Role:
Supervisor
Publication date:
1988
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
Barcode:
602835283
URN:
uuid:e5f11388-8613-442d-9a13-d20eea92bc84
Local pid:
td:602835283

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