Journal article
A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy
- Abstract:
- A switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red fluorophore with a photochromic spironaphthoxazine quencher allows the fluorescence to be controlled using visible light, via photoswitching and FRET quenching. Synthetic lipid vesicles of varying composition were imaged with an average 2.5-fold resolution enhancement, compared to the confocal images. Ratiometric detection was used to probe the membrane polarity, and domains of different lipid ordering were distinguished within the same membrane.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.7MB, Terms of use)
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- Publisher copy:
- 10.1002/cphc.202300125
Authors
- Publisher:
- Wiley
- Journal:
- ChemPhysChem More from this journal
- Volume:
- 24
- Issue:
- 12
- Article number:
- e202300125
- Publication date:
- 2023-04-19
- Acceptance date:
- 2023-03-22
- DOI:
- EISSN:
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1439-7641
- ISSN:
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1439-4235
- Language:
-
English
- Keywords:
- Pubs id:
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1334085
- Local pid:
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pubs:1334085
- Deposit date:
-
2023-04-11
Terms of use
- Copyright holder:
- Frawley et al.
- Copyright date:
- 2023
- Rights statement:
- © 2023 The Authors. ChemPhysChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Notes:
- A previous version of this manuscript has been deposited on a preprint server (https://doi.org/10.26434/chemrxiv-2023-9f4zq).
- Licence:
- CC Attribution (CC BY)
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