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Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device

Abstract:

Sequencing the genomes of individual cells enables the direct determination of genetic heterogeneity amongst cells within a population. We have developed an injection-moulded valveless microfluidic device in which single cells from colorectal cancer derived cell lines (LS174T, LS180 and RKO) and fresh colorectal tumors have been individually trapped, their genomes extracted and prepared for sequencing using multiple displacement amplification (MDA). Ninety nine percent of the DNA sequences ob...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1039/c8lc00169c

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ORCID:
0000-0002-8338-1990
Pødenphant, M More by this author
Koprowska, K More by this author
Bærlocher, L More by this author
Vulders, RCM More by this author
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Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Lab on a Chip Journal website
Volume:
18
Issue:
13
Pages:
1891-1902
Publication date:
2018-06-06
Acceptance date:
2018-04-30
DOI:
EISSN:
1473-0189
ISSN:
1473-0197
Pubs id:
pubs:857035
URN:
uri:e4bef562-748b-47c3-aca5-9572611be2a5
UUID:
uuid:e4bef562-748b-47c3-aca5-9572611be2a5
Local pid:
pubs:857035
Language:
English

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