Identification of radiation-responsive genes in vitro using a gene trap strategy predicts for modulation of expression by radiation in vivo.

Journal article

A large number of genes are known to be responsive to ionizing radiation, and there is strong evidence for the existence of inducible radiation resistance in mammalian cells. We have developed a gene trap insertional mutagenesis strategy to identify novel genes involved in responses to radiation. Using this approach, we have isolated four gene-trap integrations in embryonic stem cells. In three cases (9A, 3E and 9H) the trapped genes are radiation-inducible, and in one (7D) the gene is down-r...

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Authors: Vallis, K; Chen, Z; Stanford, W; Yu, M; Hill, R

• Publication status: Published
• Journal: Radiation research
• Volume: 157
• Issue: 1
• Pages: 8-18
• Publication date: 2002-01-01
• DOI: 10.1667/0033-7587(2002)157[0008:iorrgi]2.0.co;2
• EISSN: 1938-5404
• ISSN: 0033-7587
• Language: English
• Keywords: Amino Acid Sequence; Coculture Techniques; Humans; Embryo Transfer; beta-Galactosidase; Molecular Sequence Data; Polymerase Chain Reaction; Gene Expression Regulation; Genetic Vectors; Animals; Mutagenesis, Insertional; Mice, Knockout; Female; Recombinant Fusion Proteins; Male; Lac Operon; Germ-Line Mutation; Introns; Sequence Homology, Amino Acid; Embryo, Mammalian; Chimera; Expressed Sequence Tags; Sequence Alignment; Genes; DNA Damage; Mice; Stem Cells; Fetal Proteins; Clone Cells; Genes, Reporter