Journal article
Assessment of mitochondrial function and oxygen consumption measured during ex vivo normothermic machine perfusion of injured pig kidneys helps to monitor organ viability
- Abstract:
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Donor kidney assessment may improve organ utilisation. Normothermic Machine Perfusion (NMP) has the potential to facilitate this advance. The mechanism of action is not yet determined and we aimed to assess mitochondrial function during NMP. Anaesthetised pigs (n = 6) had one kidney clamped for 60 min. The healthy contralateral kidney was removed and underwent NMP for 8 h (healthy control (HC), n = 6). Following 60 min warm ischaemia the injured kidney underwent HMP for 24 h, followed by NMP for 8 h (n = 6). Mitochondria were extracted from fresh tissue for analysis. Injured kidneys were analysed as two separate groups (IMa, n = 3 and IMb, n = 3). Renal resistance was higher (0.39ï, ± 0.29 vs. 1.65ï, ± 0.85; p = 0.01) and flow was lower (55ï, ± 28 vs. 7ï, ± 4; p = 0.03) during HMP in IMb than IMa. NMP blood flow was higher in IMa versus IMb (2-way ANOVA; p < 0.001) After 60 min NMP, O2 consumption was significantly lower in IMb versus IMa (p ≤ 0.002). State-3 respiration was significantly different between the groups (37ï, ± 19 vs. 24ï, ± 14 vs. 10ï, ± 8; nmolO2/min/mg; p = 0.049). Lactate levels were significantly lower in IMa versus IMb (p = 0.028). Mitochondrial respiration levels during NMP may be suggestive of kidney viability. Oxygen consumption, renal blood flow and lactate can differentiate severity of kidney injury during NMP.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.8MB, Terms of use)
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- Publisher copy:
- 10.3389/ti.2022.10420
Authors
- Publisher:
- Frontiers Media
- Journal:
- Transplant International More from this journal
- Volume:
- 35
- Article number:
- 10420
- Publication date:
- 2022-05-31
- Acceptance date:
- 2022-05-10
- DOI:
- EISSN:
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1432-2277
- ISSN:
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0934-0874
- Pmid:
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35711321
- Language:
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English
- Keywords:
- Pubs id:
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1264427
- Local pid:
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pubs:1264427
- Deposit date:
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2024-04-23
Terms of use
- Copyright holder:
- Hunter et al.
- Copyright date:
- 2022
- Rights statement:
- Copyright © 2022 Hunter, Faro, Rozenberg, Dengu, Ogbemudia, Weissenbacher, Mulvey, Knijff, Gopalakrishnan and Ploeg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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