Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008–2017)

Isgren, CM; Edwards, T; Pinchbeck, GL; Winward, E; Adams, ER; Norton, P; Timofte, D; Maddox, TW; Clegg, PD; Williams, NJ

Journal article

Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008–2017)

Abstract:: Background: This study investigated changes over time in the epidemiology of extended-spectrum β-lactamase (ESBL) producing Escherichia coli within a single equine referral hospital in the UK. Faecal samples were collected from hospitalised horses in 2008 and 2017, processed using selective media and standard susceptibility laboratory methods. A novel real-time PCR with high resolution melt analysis was used to distinguish blaCTX-M-1 and blaCTX-M-15 within CTX-M-1 group. Results: In 2008, 457 faecal samples from 103 horses were collected, with ESBL-producing E. coli identified in 131 samples (28.7, 95% CI 24.6–33.1). In 2017, 314 faecal samples were collected from 74 horses with ESBL-producing E. coli identified in 157 samples (50.0, 95% CI 44.5–55.5). There were 135 and 187 non-duplicate ESBL-producing isolates from 2008 and 2017, respectively. In 2008, 12.6% of isolates belonged to CTX-M-1 group, all carrying blaCTX-M-1, whilst in 2017, 94.1% of isolates were CTX-M-1 group positive and of these 39.2 and 60.8% of isolates carried blaCTX-M-1 and blaCTX-M-15, respectively. In addition, the prevalence of doxycycline, gentamicin and 3rd generation cephalosporin resistance increased significantly from 2008 to 2017 while a decreased prevalence of phenotypic resistance to potentiated sulphonamides was observed. Conclusions: The real-time PCR proved a reliable and high throughput method to distinguish between blaCTX-M-1 and blaCTX-M-15. Furthermore, its use in this study demonstrated the emergence of faecal carriage of CTX-M-15 in hospitalised horses, with an increase in prevalence of ESBL-producing E. coli as well as increased antimicrobial resistance to frequently used antimicrobials. Keywords: ESBL-producing E. coli, Melt curve analysis, Multidrug resistance, High resolution melt, Real time PCR, CTX-M-1 grou

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Isgren, C. M., Edwards, T., Pinchbeck, G. L., Winward, E., Adams, E. R., Norton, P., Timofte, D., Maddox, T. W., Clegg, P. D., & Williams, N. J. (2019). Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008–2017). BMC Veterinary Research, 15(1), 268–268.

MLA Style

Isgren, CM, et al. “Emergence of Carriage of CTX-M-15 in Faecal Escherichia Coli in Horses at an Equine Hospital in the UK; Increasing Prevalence over a Decade (2008–2017).” BMC Veterinary Research, vol. 15, no. 1, 2019, pp. 268–68.

Chicago Style

Isgren, CM, T Edwards, GL Pinchbeck, et al. 2019. “Emergence of Carriage of CTX-M-15 in Faecal Escherichia Coli in Horses at an Equine Hospital in the UK; Increasing Prevalence over a Decade (2008–2017).” BMC Veterinary Research 15 (1): 268–68.
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