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Active RNA polymerases are localized within discrete transcription "factories' in human nuclei.

Abstract:

Nascent transcripts in permeabilized HeLa cells were elongated by approximately 30-2,000 nucleotides in Br-UTP or biotin-14-CTP, before incorporation sites were immunolabelled either pre- or post-embedding, and visualized by light or electron microscopy. Analogues were concentrated in approximately 2,100 (range 2,000-2,700) discrete sites attached to a nucleoskeleton and surrounded by chromatin. A typical site contained a cluster (diameter 71 nm) of at least 4, and probably about 20, engaged ...

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Publication status:
Published

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Authors


Iborra, FJ More by this author
Jackson, DA More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Journal of cell science
Volume:
109 ( Pt 6)
Pages:
1427-1436
Publication date:
1996-06-05
EISSN:
1477-9137
ISSN:
0021-9533
URN:
uuid:e1c2bffc-e192-4bc5-8816-4b9caa4e602f
Source identifiers:
19623
Local pid:
pubs:19623

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