- Abstract:
-
DNA molecules tethered inside a protein pore can be used as a tool to probe distance and electrical potential. The approach and its limitations were tested with alpha-hemolysin, a pore of known structure. A single oligonucleotide was attached to an engineered cysteine to allow the binding of complementary DNA strands inside the wide internal cavity of the extramembranous domain of the pore. The reversible binding of individual oligonucleotides produced transient current blockades in single ch...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/s0006-3495(02)75322-8
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- Journal:
- Biophysical journal
- Volume:
- 83
- Issue:
- 6
- Pages:
- 3202-3210
- Publication date:
- 2002-12-05
- DOI:
- EISSN:
-
1542-0086
- ISSN:
-
0006-3495
- URN:
-
uuid:e157e05f-e3e2-46ed-940d-f265210de6e2
- Source identifiers:
-
52210
- Local pid:
- pubs:52210
- Language:
- English
- Keywords:
- Copyright date:
- 2002
Journal article
Probing distance and electrical potential within a protein pore with tethered DNA.
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