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Journal article

Probing distance and electrical potential within a protein pore with tethered DNA.

Abstract:

DNA molecules tethered inside a protein pore can be used as a tool to probe distance and electrical potential. The approach and its limitations were tested with alpha-hemolysin, a pore of known structure. A single oligonucleotide was attached to an engineered cysteine to allow the binding of complementary DNA strands inside the wide internal cavity of the extramembranous domain of the pore. The reversible binding of individual oligonucleotides produced transient current blockades in single ch...

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Publication status:
Published

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Journal:
Biophysical journal
Volume:
83
Issue:
6
Pages:
3202-3210
Publication date:
2002-12-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:e157e05f-e3e2-46ed-940d-f265210de6e2
Source identifiers:
52210
Local pid:
pubs:52210

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