Journal article
Identifying key membrane protein lipid interactions using mass spectrometry
- Abstract:
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With the recent success in determining membrane protein structures, further detailed understanding of the identity and function of the bound lipidome is essential. Using an approach that combines high-energy native mass spectrometry (HE-nMS) and solution-phase lipid profiling, this protocol can be used to determine the identity of the endogenous lipids that directly interact with a protein. Furthermore, this method can identify systems in which such lipid binding has a major role in regulatin...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
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(Preview, Accepted manuscript, pdf, 406.3KB, Terms of use)
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- Publisher copy:
- 10.1038/nprot.2018.014
Authors
Funding
Bibliographic Details
- Publisher:
- Nature Publishing Group
- Journal:
- Nature Protocols More from this journal
- Volume:
- 13
- Issue:
- 5
- Pages:
- 1106–1120
- Publication date:
- 2018-04-26
- DOI:
- EISSN:
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1750-2799
- ISSN:
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1754-2189
- Pmid:
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29700483
Item Description
- Language:
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English
- Pubs id:
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pubs:846048
- UUID:
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uuid:e1012d99-5619-4f46-8e31-e0d5ffa57e58
- Local pid:
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pubs:846048
- Source identifiers:
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846048
- Deposit date:
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2018-06-11
Terms of use
- Copyright holder:
- Gupta, et al
- Copyright date:
- 2018
- Notes:
- © 2018 Gupta, et al. This is the author accepted manuscript following peer review version of the article. The final version is available online from Nature Publishing Group at: 10.1038/nprot.2018.014
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