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Large-scale association analysis of TNF/LTA gene region polymorphisms in type 2 diabetes

Abstract:

Background: The TNF/LTA locus has been a long-standing T2D candidate gene. Several studies have examined association of TNF/LTA SNPs with T2D but the majority have been small-scale and produced no convincing evidence of association. The purpose of this study is to examine T2D association of tag SNPs in the TNF/LTA region capturing the majority of common variation in a large-scale sample set of UK/Irish origin.

Methods: This study comprised a case-control (1520 cases and 2570 control samples) and a family-based component (423 parent-offspring trios). Eleven tag SNPs (rs928815, rs909253, rs746868, rs1041981 (T60N), rs1800750, rs1800629 (G-308A), rs361525 (G-238A), rs3093662, rs3093664, rs3093665, and rs3093668) were selected across the TNF/LTA locus and genotyped using a fluorescence-based competitive allele specific assay. Quality control of the obtained genotypes was performed prior to single- and multi-point association analyses under the additive model.

Results: We did not find any consistent SNP associations with T2D in the case-control or family-based datasets.

Conclusions: The present study, designed to analyse a set of tag SNPs specifically selected to capture the majority of common variation in the TNF/LTA gene region, found no robust evidence for association with T2D. To investigate the presence of smaller effects of TNF/LTA gene variation with T2D, a large-scale meta-analysis will be required.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/1471-2350-11-69

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author


Publisher:
BioMed Central
Journal:
BMC Medical Genetics More from this journal
Volume:
11
Article number:
69
Publication date:
2010-05-06
Acceptance date:
2010-05-06
DOI:
EISSN:
1471-2350


Language:
English
Keywords:
UUID:
uuid:e0d495ee-393a-4326-91ef-747378a99443
Local pid:
pubs:60599
Source identifiers:
60599
Deposit date:
2012-12-19

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