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Activity-Based Protein Profiling (ABPP) for cellular deubiquitinase (DUB) and inhibitor profiling at deep and high-throughput levels

Abstract:: This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible).

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Jones, H. B. L., Heilig, R., Kessler, B. M., & Pinto-Fernández, A. (2022). Activity-Based Protein Profiling (ABPP) for cellular deubiquitinase (DUB) and inhibitor profiling at deep and high-throughput levels. In Deubiquitinases: Methods and Protocols (pp. 101–122). Springer.

MLA Style

Jones, H. BL, et al. “Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels.” Deubiquitinases: Methods and Protocols, Springer, 2022, pp. 101–22.

Chicago Style

Jones, HBL, R Heilig, BM Kessler, and A Pinto-Fernández. 2022. “Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels.” In Deubiquitinases: Methods and Protocols, 101–22. Methods in Molecular Biology. Springer.
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Publisher copy:: 10.1007/978-1-0716-2803-4_7

Authors

+ Jones, HBL More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author

+ Heilig, R More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author

+ Kessler, BM More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author
ORCID:: 0000-0002-8160-2446

+ Pinto-Fernández, A More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author

Publisher:: Springer
Host title:: Deubiquitinases: Methods and Protocols
Pages:: 101-122
Chapter number:: 7
Series:: Methods in Molecular Biology
Series number:: 2591
Publication date:: 2022-11-10
Acceptance date:: 2022-09-30
DOI:: 10.1007/978-1-0716-2803-4_7
EISSN:: 1940-6029
ISSN:: 1064-3745

Language:: English
Keywords:: uniquitin

activitomics

chemical biology

activity-based probes

drug discovery

SBTMR

deubiquitinase enzymes

pharmacology

proteomics
Pubs id:: 1301821
Local pid:: pubs:1301821
Deposit date:: 2022-11-11

Terms of use

Copyright holder:: Jones et al.
Copyright date:: 2022
Rights statement:: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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