Measurement of myofilament-localised calcium dynamics in adult cardiomyocytes and the effect of hypertrophic cardiomyopathy mutations
Rationale: Subcellular Ca2+ indicators have yet to be developed for the myofilament where disease mutation, or small molecules may alter contractility through myofilament Ca2+ sensitivity. Here we develop and characterise genetically encoded Ca2+ indicators restricted to the myofilament to directly visualise Ca2 changes in the sarcomere.
Objective: To produce and validate myofilament restricted Ca2+ imaging probes in an adenoviral transduction adult...Expand abstract
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- Peer reviewed
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- American Heart Association Publisher's website
- Circulation Research Journal website
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- Sparrow et al.
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- Copyright © 2019 The Authors. Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.