- Abstract:
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TransRate is a tool for reference-free quality assessment of de novo transcriptome assemblies. Using only the sequenced reads and the assembly as input, we show multiple common artifacts of de novo transcriptome assembly can be readily detected. These include chimeras, structural errors, incomplete assembly and base errors. TransRate evaluates these errors to produce a diagnostic quality score for each contig and these contig scores are integrated to evaluate whole assemblies. Thus TransRate ...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted manuscript
- Files:
-
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(pdf, 1.1MB)
-
- Publisher copy:
- 10.1101/gr.196469.115
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- Publisher:
- Cold Spring Harbor Laboratory Press Publisher's website
- Journal:
- Genome Research Journal website
- Pages:
- gr.196469.115-gr.196469.115
- Publication date:
- 2016-06-05
- DOI:
- EISSN:
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1549-5469
- ISSN:
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1088-9051
- URN:
-
uuid:dfcb0f24-06fa-4510-90ff-082a8ce61ed9
- Source identifiers:
-
628076
- Local pid:
- pubs:628076
- Language:
- English
- Keywords:
- Copyright holder:
- Kelly et al
- Copyright date:
- 2016
- Notes:
- This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International license), as described at http://creativecommons.org/licenses/by/4.0/.
Journal article
TransRate: reference free quality assessment of de novo transcriptome assemblies.
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Millennium Seed Bank
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Biotechnology and Biological Sciences Research Council
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Department for International Development
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Bill and Melinda Gates Foundation
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