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Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging.

Abstract:

Interferometric scattering microscopy (iSCAT) is a light scattering-based imaging modality that offers a unique combination of imaging speed and precision for tracking nanoscopic labels and enables label-free optical sensing down to the single-molecule level. In contrast to fluorescence, iSCAT does not suffer from limitations associated with dye photochemistry and photophysics, or the requirement for fluorescent labeling. Here we present a protocol for constructing an iSCAT microscope from co...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/nprot.2016.022

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Department:
Oxford, MPLS, Chemistry, Physical & Theoretical Chem
Role:
Author
Publisher:
Nature Publisher's website
Journal:
Nature protocols Journal website
Issue:
4
Publication date:
2016-04-05
DOI:
ISSN:
1750-2799 and 1754-2189
Pubs id:
pubs:609021
URN:
uri:df81b4d0-a1ef-42b9-89f9-19111d9a0be6
UUID:
uuid:df81b4d0-a1ef-42b9-89f9-19111d9a0be6
Local pid:
pubs:609021

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