Journal article
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging.
- Abstract:
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Interferometric scattering microscopy (iSCAT) is a light scattering-based imaging modality that offers a unique combination of imaging speed and precision for tracking nanoscopic labels and enables label-free optical sensing down to the single-molecule level. In contrast to fluorescence, iSCAT does not suffer from limitations associated with dye photochemistry and photophysics, or the requirement for fluorescent labeling. Here we present a protocol for constructing an iSCAT microscope from co...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Accepted manuscript, pdf, 5.7MB)
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- Publisher copy:
- 10.1038/nprot.2016.022
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Authors
Bibliographic Details
- Publisher:
- Nature Publisher's website
- Journal:
- Nature protocols Journal website
- Issue:
- 4
- Publication date:
- 2016-04-01
- DOI:
- ISSN:
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1754-2189 and 1750-2799
- Pmid:
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26938114
- Source identifiers:
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609021
Item Description
- Language:
- English
- Keywords:
- Pubs id:
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pubs:609021
- UUID:
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uuid:df81b4d0-a1ef-42b9-89f9-19111d9a0be6
- Local pid:
- pubs:609021
- Deposit date:
- 2016-08-27
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- Copyright holder:
- © 2016 Nature America, Inc All rights reserved
- Copyright date:
- 2016
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