- Abstract:
-
Interferometric scattering microscopy (iSCAT) is a light scattering-based imaging modality that offers a unique combination of imaging speed and precision for tracking nanoscopic labels and enables label-free optical sensing down to the single-molecule level. In contrast to fluorescence, iSCAT does not suffer from limitations associated with dye photochemistry and photophysics, or the requirement for fluorescent labeling. Here we present a protocol for constructing an iSCAT microscope from co...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted Manuscript
- Files:
-
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(pdf, 5.7MB)
-
- Publisher copy:
- 10.1038/nprot.2016.022
-
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- Publisher:
- Nature Publisher's website
- Journal:
- Nature protocols Journal website
- Issue:
- 4
- Publication date:
- 2016-04-05
- DOI:
- ISSN:
-
1750-2799 and 1754-2189
- Pubs id:
-
pubs:609021
- URN:
-
uri:df81b4d0-a1ef-42b9-89f9-19111d9a0be6
- UUID:
-
uuid:df81b4d0-a1ef-42b9-89f9-19111d9a0be6
- Local pid:
- pubs:609021
- Language:
- English
- Keywords:
- Copyright holder:
- © 2016 Nature America, Inc. All rights reserved.
- Copyright date:
- 2016
Journal article
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging.
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