Journal article icon

Journal article

Thermodynamic and kinetic stability of intermolecular triple helices containing different proportions of C+*GC and T*AT triplets.

Abstract:

We have used oligonucleotides containing appropriately placed fluorophores and quenchers to measure the stability of 15mer intermolecular triplexes with third strands consisting of repeats of TTT, TTC, TCC and TCTC. In the presence of 200 mM sodium (pH 5.0) triplexes that contain only T.AT triplets are unstable and melt below 30 degrees C. In contrast, triplets with repeats of TTC, TCC and CTCT melt at 67, 72 and 76 degrees C, respectively. The most stable complex is generated by the sequence...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1093/nar/gkg782

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Journal:
Nucleic acids research
Volume:
31
Issue:
19
Pages:
5598-5606
Publication date:
2003-10-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:df649fc0-2440-4f7c-99d2-8830a1091533
Source identifiers:
400054
Local pid:
pubs:400054

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP