Journal article
Label-free methods for optical in vitro characterization of protein–protein interactions
- Abstract:
- Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 4.5MB, Terms of use)
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- Publisher copy:
- 10.1039/d1cp01072g
Authors
- Publisher:
- Royal Society of Chemistry
- Journal:
- Physical Chemistry Chemical Physics More from this journal
- Volume:
- 23
- Issue:
- 31
- Pages:
- 16488-16500
- Publication date:
- 2021-08-03
- Acceptance date:
- 2021-07-23
- DOI:
- EISSN:
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1463-9084
- ISSN:
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1463-9076
- Language:
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English
- Keywords:
- Pubs id:
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1189525
- Local pid:
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pubs:1189525
- Deposit date:
-
2021-08-09
- ARK identifier:
Terms of use
- Copyright holder:
- Soltermann et al.
- Copyright date:
- 2021
- Rights statement:
- Copyright © 2021 The Authors. Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
- Licence:
- CC Attribution (CC BY)
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