A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

Hanke, L; Das, H; Sheward, DJ; Perez Vidakovics, L; Urgard, E; Moliner-Morro, A; Kim, C; Karl, V; Pankow, A; Smith, NL; Porebski, B; Fernandez-Capetillo, O; Sezgin, E; Pedersen, GK; Coquet, JM; Hällberg, BM; Murrell, B; McInerney, GM

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Journal article

A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

Abstract:: Experiments with replication-competent SARS-CoV-2 were performed in the Biomedicum BSL3 core facility, Karolinska Institutet. We thank Jonas Klingström for providing Calu-3 cells and sharing the Swedish SARS-CoV-2 isolate, and Alex Sigal from the Africa Health Research Institute for providing the beta variant (B.1.351/501Y.V2) isolate. We thank Penny Moore and the NICD (South Africa) for providing the B.1.351/beta variant spike plasmid, which was generated using funding from the South African Medical Research Council. We gratefully acknowledge the G2P-UK National Virology consortium funded by MRC/UKRI (grant ref: MR/W005611/1.) and the Barclay Lab at Imperial College for providing the B.1.617.2 spike plasmid. All cryo-EM data were collected in the Karolinska Institutet’s 3D-EM facility. We thank Agustin Ure for assistance with figure generation and Tomas Nyman (Protein Science Facility at KI) for providing access to SPR instruments. L.H. was supported by the David och Astrid Hageléns stiftelse, the Clas Groschinskys Minnesfond and a Jonas Söderquist’s scholarship. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 101003653 (CoroNAb), to B.M. and G.M.M. B.M.H. is supported by the Knut and Alice Wallenberg Foundation (KAW 2017.0080 and KAW 2018.0080). The work was supported by project grants from the Swedish Research Council to E.S. (2020-02682), B.M.H. (2017-6702 and 2018-3808), B.M. (2018-02381) and to G.M.M. (2018-03914 and 2018-03843). E.S. is supported by Karolinska Institutet Foundation Grants, National Molecular Medicine Program Grants, and the grants from the SciLifeLab National COVID-19 Research Program, financed by the Knut and Alice Wallenberg Foundation. We thank National Microscopy Infrastructure, NMI (VR-RFI 2016-00968).N

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Hanke, L., Das, H., Sheward, D. J., Perez Vidakovics, L., Urgard, E., Moliner-Morro, A., Kim, C., Karl, V., Pankow, A., Smith, N. L., Porebski, B., Fernandez-Capetillo, O., Sezgin, E., Pedersen, G. K., Coquet, J. M., Hällberg, B. M., Murrell, B., & McInerney, G. M. (2022). A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo. Nature Communications, 13(1), 155–155.

MLA Style

Hanke, L, et al. “A Bispecific Monomeric Nanobody Induces Spike Trimer Dimers and Neutralizes SARS-CoV-2 in Vivo.” Nature Communications, vol. 13, no. 1, 2022, pp. 155–55.

Chicago Style

Hanke, L, H Das, DJ Sheward, et al. 2022. “A Bispecific Monomeric Nanobody Induces Spike Trimer Dimers and Neutralizes SARS-CoV-2 in Vivo.” Nature Communications 13 (1): 155–55.
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