Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults.

Litt, D; Samuel, D; Duncan, J; Harnden, A; George, R; Harrison, T

Journal article

Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults.

Abstract:: Bordetella pertussis infection is being increasingly recognized as a cause of prolonged, distressing cough (without whooping symptoms) in children and young adults. Diagnosis of infection in this population is important for treatment and surveillance purposes, and may also prove useful in reducing transmission to unvaccinated babies, for whom disease can be fatal. Serum IgG titres against pertussis toxin (PT) are routinely used as a marker of recent or persisting B. pertussis infection. However, collection of serum from young children is difficult, and compliance amongst these subjects to give samples is low. To circumvent these problems, an IgG-capture ELISA capable of detecting anti-PT IgG in oral fluid was devised. The assay was evaluated by comparison to a serum ELISA, using 187 matched serum and oral fluid samples from children (aged 5-16 years) with a history of prolonged coughing, whose serum anti-PT titre had already been determined (69 seropositive, 118 seronegative). The results showed that, using a cutoff of 70 arbitrary units (AU), the oral fluid assay detected seropositive subjects with a sensitivity of 79.7% [95% confidence interval (CI) 68.3-88.4] and a specificity of 96.6% (95% CI 91.5-99.1). Thus, oral fluid titres of >or=70 AU would possess a positive predictive value of 76.2-93.2% for pertussis amongst children with chronic coughs when used as a surrogate for the serum ELISA (assuming disease prevalence of 12-37%). This oral fluid ELISA will greatly assist in the convenience of B. pertussis disease diagnosis and surveillance.

Publication status:: Published

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Cite

Cite this record

APA Style

Litt, D., Samuel, D., Duncan, J., Harnden, A., George, R., & Harrison, T. (2006). Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in children and young adults. Journal of Medical Microbiology, 55(Pt 9), 1223–1228.

MLA Style

Litt, D., et al. “Detection of Anti-Pertussis Toxin IgG in Oral Fluids for Use in Diagnosis and Surveillance of Bordetella Pertussis Infection in Children and Young Adults.” Journal of Medical Microbiology, vol. 55, no. Pt 9, 2006, pp. 1223–28.

Chicago Style

Litt, D, D Samuel, J Duncan, A Harnden, R George, and T Harrison. 2006. “Detection of Anti-Pertussis Toxin IgG in Oral Fluids for Use in Diagnosis and Surveillance of Bordetella Pertussis Infection in Children and Young Adults.” Journal of Medical Microbiology 55 (Pt 9): 1223–28.
Share
Print