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Maternal colonisation with Streptococcus agalactiae, and associated stillbirth and neonatal disease in coastal Kenya

Abstract:
Streptococcus agalactiae (Group B Streptococcus, GBS) causes neonatal disease and stillbirth, but its burden in sub-Saharan Africa is uncertain. We assessed maternal recto-vaginal GBS colonisation (7967 women), stillbirth and neonatal disease. Whole genome sequencing was used to determine serotypes, sequence types (ST), and phylogeny. We found low maternal GBS colonisation prevalence (934/7967, 12%), but comparatively high incidence of GBS associated stillbirth and early onset neonatal disease (EOD) in hospital (0.91(0.25-2.3)/1000 births; 0.76(0.25-1.77)/1000 live-births respectively). However, using a population denominator, EOD incidence was considerably reduced (0.13(0.07-0.21)/1000 live-births). Treated cases of EOD had very high case fatality (17/36, 47%), especially within 24 hours of birth, making under ascertainment of community-born cases highly likely, both here and in similar facility-based studies. Maternal GBS colonisation was less common in women with low socio-economic status, HIV infection and undernutrition, but when GBS-colonised, they were more likely colonised by the most virulent clone, CC17. CC17 accounted for 267/915(29%) of maternal colonising (265/267(99%) serotype III, 2/267(0.7%) serotype IV), and 51/73(70%) of neonatal disease cases (all serotype III). Trivalent (Ia/II/III) and pentavalent (Ia/Ib/II/III/V) vaccines would cover 71/73(97%) and 72/73(99%) of disease-causing serotypes respectively. Serotype IV should be considered for inclusion, with evidence of capsular switching in CC17 strains.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nmicrobiol.2016.67

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Tropical Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Tropical Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author


More from this funder
Funding agency for:
Sheppard, A
Walker, A
Crook, D
Grant:
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
More from this funder
Funding agency for:
Sheppard, A
Walker, A
Crook, D
Grant:
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
More from this funder
Funding agency for:
Sheppard, A
Walker, A
Crook, D
Grant:
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
More from this funder
Funding agency for:
Sheppard, A
Walker, A
Crook, D
Grant:
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
More from this funder
Funding agency for:
Berkley, J
Seale, A
Sheppard, A
Sheppard, A
Walker, A
Walker, A
Crook, D
Crook, D
Grant:
098532
093804
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
087646/Z/08/Z
077092


Publisher:
Nature Publishing Group
Journal:
Nature Microbiology More from this journal
Volume:
67
Article number:
16067
Publication date:
2016-01-01
DOI:
EISSN:
2058-5276


Keywords:
Pubs id:
pubs:616612
UUID:
uuid:de0cb1f5-9618-4eb5-8db8-a82255fb60c7
Local pid:
pubs:616612
Source identifiers:
616612
Deposit date:
2016-04-19

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