Journal article : Review
From sequence to function: bridging single-molecule kinetics and molecular diversity
- Abstract:
- Biological function is fundamentally determined by nucleic acid and protein sequence. Beyond encoding genetic information, nucleic acids also display complex physicochemical parameters that shape structure, dynamics, and interactions. Understanding how sequence variation sculpts the energetic landscapes underlying these properties requires methods that capture both molecular diversity and dynamic behavior. Single-molecule techniques are ideally suited to this task, but conventional formats remain time and cost intensive. Recent breakthroughs have enabled highly multiplexed approaches for observing molecular dynamics across millions of individual molecules representing thousands of sequences or barcoded entities. Though still in development, these methods have begun to bridge sequence, structure, dynamics, and function at scale, opening new opportunities in drug discovery, molecular diagnostics, and functional genomics.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 3.4MB, Terms of use)
-
- Publisher copy:
- 10.1126/science.adv4503
Authors
+ Wellcome Trust
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- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- 110164/Z/15/Z
+ Leverhulme Trust
More from this funder
- Funder identifier:
- https://ror.org/012mzw131
- Grant:
- RPG-2024-037
+ European Research Council
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- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- ERC-ADG-101092623
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 391
- Issue:
- 6784
- Pages:
- 458-465
- Publication date:
- 2026-01-29
- Acceptance date:
- 2025-12-09
- DOI:
- EISSN:
-
1095-9203
- ISSN:
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0036-8075
- Pmid:
-
41610253
- Language:
-
English
- Keywords:
- Subtype:
-
Review
- Pubs id:
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2365868
- Local pid:
-
pubs:2365868
- Deposit date:
-
2026-02-23
- ARK identifier:
Terms of use
- Copyright holder:
- Kapanidis et al.
- Copyright date:
- 2026
- Rights statement:
- Copyright © 2026 the authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
- Notes:
- The author accepted manuscript (AAM) of this paper has been made available under the University of Oxford's Open Access Publications Policy, and a CC BY public copyright licence has been applied.
- Licence:
- CC Attribution (CC BY)
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