Cell-free transcription and translation of Na,K-ATPase alpha and beta subunit cDNAs.

Journal article

Synthetic mRNAs (i.e. cRNA alpha and cRNA beta) were obtained by cell-free transcription of M13 KS(+) (Bluescript) expression vectors which contained the entire coding region of the alpha or beta subunits of lamb kidney Na,K-ATPase. Translation in reticulocyte lysates of cRNA alpha yielded full length alpha polypeptide, as well as a limited array of immunoprecipitable lower molecular weight products. cRNA beta yielded a single immunoprecipitable full length polypeptide. Association of the alp...

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Authors: Cayanis, E; Bayley, H; Edelman, I

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 265
• Issue: 19
• Pages: 10829-10835
• Publication date: 1990-07-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Receptors, Cytoplasmic and Nuclear; RNA; Dogs; Endoplasmic Reticulum; Signal Transduction; Intracellular Membranes; Molecular Weight; Ethylmaleimide; Transcription, Genetic; Sheep; Glycosylation; Sodium-Potassium-Exchanging ATPase; Hydrogen-Ion Concentration; Receptors, Peptide; Protein Biosynthesis; RNA, Complementary; Acetylglucosaminidase; Receptors, Cell Surface; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; Pancreas; Macromolecular Substances; DNA; Animals; Immunosorbent Techniques; Trypsin; Cell-Free System; Rabbits