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Cell-free transcription and translation of Na,K-ATPase alpha and beta subunit cDNAs.

Abstract:

Synthetic mRNAs (i.e. cRNA alpha and cRNA beta) were obtained by cell-free transcription of M13 KS(+) (Bluescript) expression vectors which contained the entire coding region of the alpha or beta subunits of lamb kidney Na,K-ATPase. Translation in reticulocyte lysates of cRNA alpha yielded full length alpha polypeptide, as well as a limited array of immunoprecipitable lower molecular weight products. cRNA beta yielded a single immunoprecipitable full length polypeptide. Association of the alp...

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Publication status:
Published

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Authors


Cayanis, E More by this author
Edelman, IS More by this author
Journal:
The Journal of biological chemistry
Volume:
265
Issue:
19
Pages:
10829-10835
Publication date:
1990-07-05
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:dc0d5554-8f05-412d-9fc0-e4fc8a9e3920
Source identifiers:
52242
Local pid:
pubs:52242

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