Journal article
Advances and perspectives in tissue clearing using CLARITY
- Abstract:
- CLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3D-reconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 3.3MB, Terms of use)
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- Publisher copy:
- 10.1016/j.jchemneu.2017.07.005
Authors
- Publisher:
- Elsevier
- Journal:
- Journal of Chemical Neuroanatomy More from this journal
- Volume:
- 86
- Pages:
- 19-34
- Publication date:
- 2017-07-17
- Acceptance date:
- 2017-07-12
- DOI:
- EISSN:
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1873-6300
- ISSN:
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0891-0618
- Pubs id:
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pubs:743423
- UUID:
-
uuid:db6cce8c-33eb-4d99-a3c1-fc0575fab499
- Local pid:
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pubs:743423
- Source identifiers:
-
743423
- Deposit date:
-
2017-11-13
Terms of use
- Copyright holder:
- © 2017 Elsevier BV All rights reserved
- Copyright date:
- 2017
- Notes:
- This is the author accepted manuscript following peer review version of the article. The final version is available online from Elsevier at: 10.1016/j.jchemneu.2017.07.005
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