Journal article
SIMcheck: a toolbox for successful super-resolution SIM imaging
- Abstract:
- Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms and will promote routine application of super-resolution SIM imaging in cell biology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 7.9MB, Terms of use)
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- Publisher copy:
- 10.1038/srep15915
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Volume:
- 5
- Article number:
- 15915
- Publication date:
- 2015-11-03
- DOI:
- ISSN:
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2045-2322
- Pubs id:
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pubs:572637
- UUID:
-
uuid:dafd699e-0d0e-4646-aef5-8ce13382613f
- Local pid:
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pubs:572637
- Source identifiers:
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572637
- Deposit date:
-
2015-11-12
- ARK identifier:
Terms of use
- Copyright holder:
- Ball et al
- Copyright date:
- 2015
- Notes:
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
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