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Journal article

Estimate your dose: RADDOSE-3D

Abstract:
We present the current status of RADDOSE-3D, a software tool allowing the estimation of the dose absorbed in a macromolecular crystallography diffraction experiment. The code allows a temporal and spatial dose contour map to be calculated for a crystal of any geometry and size as it is rotated in an X-ray beam, and gives several summary dose values: among them diffraction weighted dose. This allows experimenters to plan data collections which will minimise radiation damage effects by spreading the absorbed dose more homogeneously, and thus to optimise the use of their crystals. It also allows quantitative comparisons between different radiation damage studies, giving a universal 'x-axis' against which to plot various metrics. This article is protected by copyright. All rights reserved.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/pro.3302

Authors


More by this author
Institution:
University of Oxford
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author


Publisher:
Wiley
Journal:
Protein Science More from this journal
Volume:
27
Issue:
1
Pages:
217-228
Publication date:
2017-09-01
Acceptance date:
2017-09-11
DOI:
ISSN:
0961-8368 and 1469-896X
Pmid:
28921782


Language:
English
Keywords:
Pubs id:
pubs:730131
UUID:
uuid:daf0b8e6-fbbb-45f1-8cdf-611e63dfefae
Local pid:
pubs:730131
Source identifiers:
730131
Deposit date:
2017-10-09

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