Journal article
A compact monomeric intermediate identified by NMR in the denaturation of dimeric triose phosphate isomerase.
- Abstract:
-
The denaturation of triose phosphate isomerase (TIM) from Saccharomyces cerevisiae by guanidine hydrochlorids at pH 7.2 has been monitored by NMR spectroscopy in conjunction with optical spectroscopy. In the absence of denaturant, the hydrodynamic radius of 29.6(+/-0.25) A and the substantial chemical shift dispersion evident in the NMR spectrum are consistent with the highly structured dimeric native state of the protein. On the addition of 2. 2 M guanidine hydrochloride the effective hydrod...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of molecular biology
- Volume:
- 300
- Issue:
- 1
- Pages:
- 11-16
- Publication date:
- 2000-06-01
- DOI:
- EISSN:
-
1089-8638
- ISSN:
-
0022-2836
- Source identifiers:
-
31705
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:31705
- UUID:
-
uuid:d9b137f2-bcad-4170-99f3-2bd58083a353
- Local pid:
- pubs:31705
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2000
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