Increases in Na/K pump numbers in isolated human lymphocytes exposed to lithium in vitro. Reversal by myo-inositol and by inhibitors of protein kinase C and the Na/H antiport.

Journal article

Lithium (1-8 mM) caused a dose-dependent increase in the number of [3H]ouabain binding sites and in sodium/potassium (Na/K) pump activity in normal lymphocytes after incubation for 72 h. The increase in Na/K pump activity was due to an increase in the Vmax of the pump, with no change in the apparent affinity (Km) for potassium (rubidium). There was no change in the turnover number of the pump and the intracellular sodium concentration fell. The increase in [3H]ouabain binding sites was preven...

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Authors: Jenkins, R; Aronson, J; Brearley, C

• Publication status: Published
• Journal: Biochimica et biophysica acta
• Volume: 1092
• Issue: 2
• Pages: 138-144
• Publication date: 1991-04-01
• DOI: 10.1016/0167-4889(91)90147-p
• ISSN: 0006-3002
• Language: English
• Keywords: Rubidium; Female; Alkaloids; Sodium-Hydrogen Antiporter; Potassium; Protein Kinase C; Lymphocytes; Amiloride; Inositol; Carrier Proteins; Male; Sodium-Potassium-Exchanging ATPase; Staurosporine; Ouabain; Humans; Lithium; Adult