Targeting gene expression to hypoxic tumor cells.

Journal article

Solid tumors with areas of low oxygen tension (hypoxia) have a poor prognosis, as cells in this environment often survive radiation and chemotherapy. In this report we describe how this hypoxic environment can be used to activate heterologous gene expression driven by a hypoxia-responsive element (HRE), which interacts with the transcriptional complex hypoxia-inducible factor-1 (HIF-1). Our results demonstrate that the HIF-1/HRE system of gene regulation is active in hypoxic tumor cells and s...

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Authors: Dachs, G; Patterson, A; Firth, J; Ratcliffe, P; Townsend, K

• Publication status: Published
• Journal: Nature medicine
• Volume: 3
• Issue: 5
• Pages: 515-520
• Publication date: 1997-05-01
• DOI: 10.1038/nm0597-515
• EISSN: 1546-170X
• ISSN: 1078-8956
• Language: English
• Keywords: Phosphoglycerate Kinase; Neoplasm Transplantation; Mice, Nude; Antimetabolites, Antineoplastic; Humans; Fluorouracil; Transcription Factors; Misonidazole; Mice; Fibrosarcoma; Promoter Regions, Genetic; Hypoxia-Inducible Factor 1, alpha Subunit; Cytosine Deaminase; Nucleoside Deaminases; Recombinant Fusion Proteins; DNA-Binding Proteins; Oxygen; Cell Hypoxia; Genes, Reporter; Prodrugs; Nuclear Proteins; Gene Expression Regulation, Neoplastic; Hypoxia-Inducible Factor 1; Flucytosine; Animals; Tumor Cells, Cultured