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SARS-CoV-2 within-host diversity and transmission

Abstract:
Extensive global sampling and sequencing of the pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have enabled researchers to monitor its spread and to identify concerning new variants. Two important determinants of variant spread are how frequently they arise within individuals and how likely they are to be transmitted. To characterize within-host diversity and transmission, we deep-sequenced 1313 clinical samples from the United Kingdom. SARS-CoV-2 infections are characterized by low levels of within-host diversity when viral loads are high and by a narrow bottleneck at transmission. Most variants are either lost or occasionally fixed at the point of transmission, with minimal persistence of shared diversity, patterns that are readily observable on the phylogenetic tree. Our results suggest that transmission-enhancing and/or immune-escape SARS-CoV-2 variants are likely to arise infrequently but could spread rapidly if successfully transmitted.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1126/science.abg0821

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Big Data Institute
Role:
Author
ORCID:
0000-0002-7089-7680
More by this author
Role:
Author
ORCID:
0000-0002-2671-3864
More by this author
Role:
Author
ORCID:
0000-0001-7578-7301
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
ORCID:
0000-0002-9847-1526


Publisher:
American Association for the Advancement of Science
Journal:
Science More from this journal
Volume:
372
Issue:
6539
Article number:
eabg0821
Place of publication:
United States
Publication date:
2021-03-09
Acceptance date:
2021-03-03
DOI:
EISSN:
1095-9203
ISSN:
0036-8075


Language:
English
Keywords:
Pubs id:
1167634
Local pid:
pubs:1167634
Deposit date:
2021-03-24
ARK identifier:

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