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13CHD2 methyl group probes of millisecond time scale exchange in proteins by 1H relaxation dispersion: an application to proteasome gating residue dynamics.

Abstract:

A pulse scheme is presented for quantifying millisecond time scale chemical exchange processes in proteins by measuring (1)H CPMG relaxation dispersion profiles of (13)CHD(2) methyl groups. The use of (13)CHD(2) isotopomers for (1)H methyl dispersion experiments eliminates problems with interconversion between differentially relaxing proton transitions that complicate the extraction of accurate exchange parameters when (13)CH(3) probes are used. Good agreement is demonstrated between extracte...

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Publication status:
Published

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Publisher copy:
10.1021/ja104578n

Authors


Baldwin, AJ More by this author
Religa, TL More by this author
Hansen, DF More by this author
Bouvignies, G More by this author
Journal:
Journal of the American Chemical Society
Volume:
132
Issue:
32
Pages:
10992-10995
Publication date:
2010-08-05
DOI:
EISSN:
1520-5126
ISSN:
0002-7863
URN:
uuid:d9278129-bc31-452f-b0ba-71c89a43c79b
Source identifiers:
386643
Local pid:
pubs:386643

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