Journal article
Simultaneous viral whole-genome sequencing and differential expression profiling in respiratory syncytial virus infection of infants
- Abstract:
- Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between two RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
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(Preview, Accepted manuscript, pdf, 2.3MB, Terms of use)
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- Publisher copy:
- 10.1093/infdis/jiaa448
- Publisher:
- Oxford University Press
- Journal:
- Journal of Infectious Diseases More from this journal
- Volume:
- 222
- Issue:
- S7
- Pages:
- S666-S671
- Publication date:
- 2020-07-23
- Acceptance date:
- 2020-07-15
- DOI:
- EISSN:
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1537-6613
- ISSN:
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0022-1899
- Pmid:
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32702120
- Language:
-
English
- Keywords:
- Pubs id:
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1122260
- Local pid:
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pubs:1122260
- Deposit date:
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2020-07-30
Terms of use
- Copyright holder:
- Lin et al.
- Copyright date:
- 2020
- Rights statement:
- © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.
- Notes:
- This is the accepted manuscript version of the article. The final published version is available from OUP at https://doi.org/10.1093/infdis/jiaa448
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