- Abstract:
-
A method to produce alphabeta T-cell receptors (TCRs) in a soluble form suitable for biophysical analysis was devised involving in vitro refolding of a TCR fusion protein. Polypeptides corresponding to the variable and constant domains of each chain of a human and a murine receptor, fused to a coiled coil heterodimerization motif from either c-Jun (alpha) or v-Fos (beta), were overexpressed separately in Escherichia coli. Following recovery from inclusion bodies, the two chains of each recept...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1110/ps.8.11.2418
-
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- Journal:
- Protein science : a publication of the Protein Society
- Volume:
- 8
- Issue:
- 11
- Pages:
- 2418-2423
- Publication date:
- 1999-11-05
- DOI:
- EISSN:
-
1469-896X
- ISSN:
-
0961-8368
- URN:
-
uuid:d7353f16-b641-4cd1-a347-17c1b50bccef
- Source identifiers:
-
29642
- Local pid:
- pubs:29642
- Language:
- English
- Keywords:
- Copyright date:
- 1999
Journal article
Production of soluble alphabeta T-cell receptor heterodimers suitable for biophysical analysis of ligand binding.
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