Journal article icon

Journal article

Production of soluble alphabeta T-cell receptor heterodimers suitable for biophysical analysis of ligand binding.

Abstract:

A method to produce alphabeta T-cell receptors (TCRs) in a soluble form suitable for biophysical analysis was devised involving in vitro refolding of a TCR fusion protein. Polypeptides corresponding to the variable and constant domains of each chain of a human and a murine receptor, fused to a coiled coil heterodimerization motif from either c-Jun (alpha) or v-Fos (beta), were overexpressed separately in Escherichia coli. Following recovery from inclusion bodies, the two chains of each recept...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1110/ps.8.11.2418

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, CCMP
Role:
Author
Expand authors...
Journal:
Protein science : a publication of the Protein Society
Volume:
8
Issue:
11
Pages:
2418-2423
Publication date:
1999-11-05
DOI:
EISSN:
1469-896X
ISSN:
0961-8368
URN:
uuid:d7353f16-b641-4cd1-a347-17c1b50bccef
Source identifiers:
29642
Local pid:
pubs:29642

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP