Analysis of protein complexes in wheat amyloplasts reveals functional interactions among starch biosynthetic enzymes.

Journal article

Protein-protein interactions among enzymes of amylopectin biosynthesis were investigated in developing wheat (Triticum aestivum) endosperm. Physical interactions between starch branching enzymes (SBEs) and starch synthases (SSs) were identified from endosperm amyloplasts during the active phase of starch deposition in the developing grain using immunoprecipitation and cross-linking strategies. Coimmunoprecipitation experiments using peptide-specific antibodies indicate that at least two disti...

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Authors: Tetlow, I; Beisel, K; Cameron, S; Makhmoudova, A; Liu, F

• Publication status: Published
• Journal: Plant physiology
• Volume: 146
• Issue: 4
• Pages: 1878-1891
• Publication date: 2008-04-01
• DOI: 10.1104/pp.108.116244
• EISSN: 1532-2548
• ISSN: 0032-0889
• Language: English
• Keywords: Triticum; Mass Spectrometry; Enzymes; Glucans; Plant Proteins; Phosphorylation; Electrophoresis, Polyacrylamide Gel; Chromatography, Gel; Starch; Immunoprecipitation; Amino Acid Sequence; Molecular Sequence Data