Journal article
Propagation of human prostate tissue from induced pluripotent stem cells
- Abstract:
- Primary culture of human prostate organoids and patient‐derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complimentary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 22.7MB, Terms of use)
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- Publisher copy:
- 10.1002/sctm.19-0286
Authors
- Publisher:
- Wiley
- Journal:
- Stem Cells Translational Medicine More from this journal
- Volume:
- 9
- Issue:
- 7
- Pages:
- 734–745
- Publication date:
- 2020-03-14
- Acceptance date:
- 2020-01-29
- DOI:
- EISSN:
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2157-6580
- ISSN:
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2157-6564
- Pmid:
-
32170918
- Language:
-
English
- Keywords:
- Pubs id:
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1093783
- Local pid:
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pubs:1093783
- Deposit date:
-
2020-03-15
Terms of use
- Copyright holder:
- Hepburn et al.
- Copyright date:
- 2020
- Rights statement:
- © 2020 The Authors. STEM CELLS TRANSLATIONAL MEDICINE published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. This is an open access article under the terms of the Creative Commons Attribution License
- Licence:
- CC Attribution (CC BY)
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