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Live-cell superresolution microscopy reveals the organization of RNA polymerase in the bacterial nucleoid

Abstract:

Despite the fundamental importance of transcription, a comprehensive analysis of RNA polymerase (RNAP) behavior and its role in the nucleoid organization in vivo is lacking. Here, we used superresolution microscopy to study the localization and dynamics of the transcription machinery and DNA in live bacterial cells, at both the single-molecule and the population level. We used photoactivated single-molecule tracking to discriminate between mobile RNAPs and RNAPs specifically bound to DNA, eit...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1073/pnas.1507592112

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
ORCID:
0000-0001-5685-0352
More by this author
ORCID:
0000-0002-9108-0848
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
ORCID:
0000-0002-3579-0888
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
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Publisher:
National Academy of Sciences Publisher's website
Journal:
Proceedings of the National Academy of Sciences Journal website
Volume:
112
Issue:
32
Pages:
E4390-E4399
Publication date:
2015-07-29
Acceptance date:
2015-07-02
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Pubs id:
pubs:536736
URN:
uri:d487d958-8a40-418b-9d59-05dacce4f87b
UUID:
uuid:d487d958-8a40-418b-9d59-05dacce4f87b
Local pid:
pubs:536736

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