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A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats

Abstract:
A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro, suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration. However, in vivo experiments have not been conducted. In this study, we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap. The Basso, Beattie, and Bresnahan locomotor rating scale, sciatic nerve compound muscle action potential recording, Fluoro-Gold retrograde tracing, growth related protein 43/S100 immunofluorescence staining, transmission electron microscopy, gastrocnemius muscle dry/wet weight ratio, and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath, and recovery of the electrophysiological function and motor function as autologous nerve transplantation. The conduit results were superior to those of a bulk hydrogel or silicone tube transplant. These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve. The nerve conduits have the potential as a material for repairing peripheral nerve defects.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.4103/1673-5374.350212

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Oxford college:
Linacre College
Role:
Author
ORCID:
0000-0001-7613-6041


Publisher:
Medknow Publications
Journal:
Neural Regeneration Research More from this journal
Volume:
18
Issue:
3
Pages:
657-663
Publication date:
2022-08-02
Acceptance date:
2022-06-08
DOI:
EISSN:
1876-7958
ISSN:
1673-5374
Pmid:
36018191


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