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Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch

Abstract:

Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the Escherichia coli thiM riboswitch that regulates translation. We found that the riboswitch aptamer folds into the "off" conformation independent of its ligand, but switches to the "on" conformation during transcriptional pausing near the translational start ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1073/pnas.1712983115

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Physics
Sub department:
Condensed Matter Physics
Department:
Unknown
Role:
Author
ORCID:
0000-0001-8993-3955
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Role:
Author
ORCID:
0000-0002-0867-6385
Publisher:
National Academy of Sciences
Journal:
Proceedings of the National Academy of Science of the United States of America More from this journal
Volume:
115
Issue:
2
Pages:
331-336
Publication date:
2017-12-26
Acceptance date:
2017-11-30
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
Pmid:
29279370
Language:
English
Keywords:
Pubs id:
pubs:979104
UUID:
uuid:d354051f-9a2a-4708-8417-f6329067c400
Local pid:
pubs:979104
Source identifiers:
979104
Deposit date:
2019-03-11

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