Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.

Journal article

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

Authors: Botcherby, E; Smith, C; Kohl, M; Débarre, D; Booth, M

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 109
• Issue: 8
• Pages: 2919-2924
• Publication date: 2012-02-01
• DOI: 10.1073/pnas.1111662109
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Cerebral Cortex; Neurons; Mice, Inbred C57BL; Microscopy, Fluorescence, Multiphoton; Reproducibility of Results; Imaging, Three-Dimensional; Mice; Animals