Journal article
19F-NMR reveals the role of mobile loops in product and inhibitor binding by the São Paolo metallo-β-lactamase
- Abstract:
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Resistance to β-lactam antibiotics mediated by metallo-β-lactamases (MBLs) is a growing problem. We describe the use of protein-observe 19F-NMR (PrOF NMR) to study the dynamics of the São Paulo MBL (SPM-1) from β-lactam-resistant Pseudomonas aeruginosa. Cysteinyl variants on the α3 and L3 regions, which flank the di-ZnII active site, were selectively 19F-labeled using 3-bromo-1,1,1-trifluoroacetone. The PrOF NMR results reveal roles for the mobile α3 and L3 regions in the binding of both inhi...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Supplementary materials, 3.1MB, Terms of use)
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(Preview, Version of record, pdf, 2.3MB, Terms of use)
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- Publisher copy:
- 10.1002/anie.201612185
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Bibliographic Details
- Publisher:
- Wiley
- Journal:
- Angewandte Chemie International Edition More from this journal
- Volume:
- 56
- Issue:
- 14
- Pages:
- 3862–3866
- Publication date:
- 2017-03-02
- Acceptance date:
- 2017-01-31
- DOI:
- EISSN:
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1521-3757
- ISSN:
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1433-7851
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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pubs:675633
- UUID:
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uuid:d218ee2e-bbbd-40ce-8bd9-c9bd0df99575
- Local pid:
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pubs:675633
- Source identifiers:
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675633
- Deposit date:
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2017-02-03
Terms of use
- Copyright holder:
- Abboud et al
- Copyright date:
- 2017
- Rights statement:
- © 2017 The Authors. Published by Wiley-VCH Verlag GmbH and Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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