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Journal article

Construction and characterization of a 10-fold genome equivalent rat P1-derived artificial chromosome library.

Abstract:

A rat PAC library was constructed in the vector pPAC4 from genomic DNA isolated from female Brown Norway rats. This library consists of 215,409 clones arrayed in 614,384-well microtiter plates. An average insert size of 143 kb was estimated from 217 randomly isolated clones, thus representing approximately 10-fold genome coverage. This coverage provides a very high probability that the library contains a unique sequence in genome screening. Tests on randomly selected clones demonstrated that ...

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Publication status:
Published

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Publisher copy:
10.1006/geno.1998.5319

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Journal:
Genomics
Volume:
50
Issue:
3
Pages:
306-316
Publication date:
1998-06-05
DOI:
EISSN:
1089-8646
ISSN:
0888-7543
URN:
uuid:d1b47de5-2db2-4a23-934b-f8ae8089c0a1
Source identifiers:
20911
Local pid:
pubs:20911

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