- Abstract:
-
A rat PAC library was constructed in the vector pPAC4 from genomic DNA isolated from female Brown Norway rats. This library consists of 215,409 clones arrayed in 614,384-well microtiter plates. An average insert size of 143 kb was estimated from 217 randomly isolated clones, thus representing approximately 10-fold genome coverage. This coverage provides a very high probability that the library contains a unique sequence in genome screening. Tests on randomly selected clones demonstrated that ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1006/geno.1998.5319
-
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- Journal:
- Genomics
- Volume:
- 50
- Issue:
- 3
- Pages:
- 306-316
- Publication date:
- 1998-06-05
- DOI:
- EISSN:
-
1089-8646
- ISSN:
-
0888-7543
- URN:
-
uuid:d1b47de5-2db2-4a23-934b-f8ae8089c0a1
- Source identifiers:
-
20911
- Local pid:
- pubs:20911
- Language:
- English
- Keywords:
- Copyright date:
- 1998
Journal article
Construction and characterization of a 10-fold genome equivalent rat P1-derived artificial chromosome library.
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