Journal article
The Choice of HLA-Associated Peptide Enrichment and Purification Strategy Affects Peptide Yields and Creates a Bias in Detected Sequence Repertoire
- Abstract:
-
Understanding the most appropriate workflow for biochemical human leukocyte antigen (HLA)‐associated peptide enrichment prior to ligand sequencing is essential to achieve optimal sensitivity in immunopeptidomics experiments. The use of different detergents for HLA solubilization as well as complementary workflows to separate HLA‐bound peptides from HLA protein complex components after their immunoprecipitation including HPLC, C18 cartridge, and 5 kDa filter are described. It is observed that ...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Bibliographic Details
- Publisher:
- Wiley Publisher's website
- Journal:
- Proteomics Journal website
- Volume:
- 20
- Issue:
- 12
- Article number:
- 1900401
- Publication date:
- 2020-06-02
- Acceptance date:
- 2020-04-29
- DOI:
- EISSN:
-
1615-9861
- ISSN:
-
1615-9853
- Pmid:
-
32359108
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
1103622
- Local pid:
- pubs:1103622
- Deposit date:
- 2020-06-15
Terms of use
- Copyright holder:
- WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
- Copyright date:
- 2020
- Rights statement:
- © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License
- Licence:
- CC Attribution (CC BY)
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