The HIV-1 5' LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site.

Journal article

The inactivity of the 5' long terminal repeat (LTR) poly(A) site immediately downstream of the cap site maximizes the production of HIV-1 transcripts. In this paper, we demonstrate that this inactivity is mediated by the interaction of the U1 snRNP with the major splice donor site (MSD). The inhibition of the HIV-1 poly(A) site by U1 snRNP relies on a series of delicately balanced RNA processing signals. These include the poly(A) site, the major splice donor site and the splice acceptor sites...

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Authors: Ashe, M; Pearson, L; Proudfoot, N

• Publication status: Published
• Journal: EMBO journal
• Volume: 16
• Issue: 18
• Pages: 5752-5763
• Publication date: 1997-09-01
• DOI: 10.1093/emboj/16.18.5752
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Mutagenesis, Site-Directed; HIV-1; Transcription, Genetic; Hela Cells; Promoter Regions, Genetic; HIV Long Terminal Repeat; Humans; Base Sequence; RNA Splicing; Molecular Sequence Data; Transfection; Globins; Poly A; Ribonucleoprotein, U1 Small Nuclear; Recombinant Proteins