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Integrative modelling coupled with ion mobility mass spectrometry reveals structural features of the clamp loader in complex with single-stranded DNA binding protein

Abstract:

DNA polymerase III, a decameric 420-kDa assembly, simultaneously replicates both strands of the chromosome in Escherichia coli. A subassembly of this holoenzyme, the seven-subunit clamp loader complex, is responsible for loading the sliding clamp (β2) onto DNA. Here, we use structural information derived from ion mobility mass spectrometry (IM-MS) to build three-dimensional models of one form of the full clamp loader complex, γ3δδ′ψχ (254 kDa). By probing the interaction between the clamp loa...

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Publisher copy:
10.1016/j.jmb.2013.04.006

Authors


Politis, A More by this author
Ruotolo, BT More by this author
Robinson, CV More by this author
Journal:
Journal of Molecular Biology
Volume:
425
Issue:
23
Pages:
4790-4801
Publication date:
2013-11-29
DOI:
EISSN:
1089-8638
ISSN:
0022-2836
URN:
uuid:d0920f15-52fe-4a27-a76e-7906e2c18546
Source identifiers:
440680
Local pid:
pubs:440680

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