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Reproducibility of molecular phenotypes after long-term differentiation to human iPSC-derived neurons: A multi-site omics study

Abstract:

Reproducibility in molecular and cellular studies is fundamental to scientific discovery. To establish the reproducibility of a well-defined long-term neuronal differentiation protocol, we repeated the cellular and molecular comparison of the same two iPSC lines across five distinct laboratories. Despite uncovering acceptable variability within individual laboratories, we detect poor cross-site reproducibility of the differential gene expression signature between these two lines. Factor analy...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.stemcr.2018.08.013

Authors


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Name:
National Institute for Health Research
Funding agency for:
Handel, A
Cader, M
Chintawar, S
Grant:
Biomedical Research Centre, Oxford
Biomedical Research Centre, Oxford
Biomedical Research Centre, Oxford
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Name:
European Commission
Grant:
Seventh Framework Program: 115439
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Name:
Innovative Medicines Initiative Joint Undertaking
Grant:
115439
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Name:
European Federation of Pharmaceutical Industries and Associations
Publisher:
Elsevier
Journal:
Stem Cell Reports More from this journal
Volume:
11
Issue:
4
Pages:
897-911
Publication date:
2018-09-20
Acceptance date:
2018-08-21
DOI:
EISSN:
2213-6711
Pmid:
30245212
Language:
English
Keywords:
Pubs id:
pubs:921757
UUID:
uuid:d040c80d-73f8-4612-a8b2-2a2a29660fe9
Local pid:
pubs:921757
Source identifiers:
921757
Deposit date:
2018-09-28

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