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Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.

Abstract:

We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT>GT>GG>AA approximately TT approximately TC>CA>GA>CC>GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n). Stud...

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Publication status:
Published

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Publisher copy:
10.1042/0264-6021:3540627

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Biochemical journal
Volume:
354
Issue:
Pt 3
Pages:
627-633
Publication date:
2001-03-01
DOI:
EISSN:
1470-8728
ISSN:
0264-6021
Source identifiers:
400237
Language:
English
Keywords:
Pubs id:
pubs:400237
UUID:
uuid:cf5dc2ef-8b8e-4cb4-a08f-3380c66fb9f8
Local pid:
pubs:400237
Deposit date:
2013-11-16

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