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Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean

Abstract:

It is of great significance to understand CO2 fixation in the oceans. Using single cell Raman spectra SCRS) as biochemical profiles, Raman activated cell ejection (RACE) was able to link phenotypes and genotypes of cells. Here we show that mini-metagenomic sequences from RACE can be used as a reference to reconstruct nearly complete genomes of key functional bacteria by binning shotgun metagenomic sequencing data. By applying this approach to 13C-bicarbonate spiked seawater from euphotic zon...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1111/1462-2920.14268

Authors


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Oxford University More from this funder
National Natural Science Foundation of China More from this funder
State Oceanic Administration of China More from this funder
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Publisher:
Wiley Publisher's website
Journal:
Environmental Microbiology Journal website
Volume:
20
Issue:
6
Pages:
2241-2255
Publication date:
2018-05-04
Acceptance date:
2018-05-01
DOI:
ISSN:
1462-2920 and 1462-2912
Pubs id:
pubs:845769
URN:
uri:ce6dc1d8-7fb6-43c9-ba26-4ed6dd9ea500
UUID:
uuid:ce6dc1d8-7fb6-43c9-ba26-4ed6dd9ea500
Local pid:
pubs:845769

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