Isolation and characterization of the outer-membrane proteins of Burkholderia (Pseudomonas) pseudomallei.
Membranes obtained from whole-cell lysates of Burkholderia (Pseudomonas) pseudomallei (strain 319a) were separated into four fractions by sucrose density gradient centrifugation. Membranes were characterized by enzymic and chemical analyses, and by SDS-PAGE. Cytoplasmic membranes and two forms of outer membranes (OM-1, OM-2) were detected. The major outer-membrane proteins had M(r) values of 70,000, 38,000, 31,000, 24,000 and 17,000. To determine which outer-membrane proteins were common to B...Expand abstract
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